Biochem/physiol Actions

Reversible: no

Product does not compete with ATP.

Cell permeable: yes

Primary TargetDNA topoisomerase 1

General description

A cell-permeable, reversible DNA topoisomerase I inhibitor that binds to and stabilizes the topoisomerase-DNA covalent complex. Possesses antileukemic and antitumor properties. Inhibits Tat-mediated transactivation of HIV-1. Cytostatic for non-tumorigenic cells but cytotoxic for tumorigenic cells. Induces apoptosis of HL-60 cells and mouse thymocytes.

A cell-permeable DNA topoisomerase I inhibitor. Exhibits anti-leukemic and antitumor properties. Induces apoptosis in HL-60 cells and mouse thymocytes. Arrests cells at the G₂/M phase.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.

Jones, C.B., et al. 1997. Cancer Chemother. Pharmacol.40, 475.Staron, K., et al. 1994. Carcinogenesis 15, 2953.Tanizawa, A., et al. 1994. J. Natl. Cancer Inst. 86, 836.Gorczyca, W., et al. 1993. Toxicol. Lett.67, 249.Onishi, Y., et al. 1993. Biochim. Biophys. Acta1175, 147.Pantazis, P., et al. 1993. Int. J. Cancer53, 863.Morham, S.G., and Shuman, S. 1992. J. Biol. Chem.267, 15984.Hertzberg, R.P., et al. 1990. Biochem. J.28, 4629.Hertzberg, R.P., et al. 1990. J. Biol. Chem.265, 19287.Hsiang, Y.H., et al. 1985. J. Biol. Chem.260, 14873.

Packaging

50 mg in Alu drum

Preparation Note

Stock solutions made with methanol will remain cloudy.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 2 months at -20°C.

Warning

Toxicity: Toxic & Carcinogenic / Teratogenic (G)